SMOOTH MUSCLE (ISOLATED ORGANS)

Ref ML1

Species : Guinea pig

Contractile activity of isolated segments of ileum is recorded under isometric conditions.

Isolated preparations of guinea pig ileum are used for evaluation of both neurotropic (muscarinic, histaminic, serotoninergic, nicotinic, bradykinin, cholecystokinin) and musculotropic spasmolytic or spasmogenic effects.

• Cholinergic, histaminergic, serotoninergic or nicotinic agonist or antagonist, or bradykinin, cholecystokinin or LTD4 antagonist effect
• Musculotropic spasmogenic or spasmolytic effect

Reference substances: Atropine, BaCl₂, Bradykinin, Carbachol, Cholecystokinin, Hexamethonium, Histamine, LTD4, Nicergoline, Nicotine, Papaverine, Pyrilamine, Serotonin (in alphabetical order).

Ref ML2

Species : Rat

Spontaneous contractions of isolated duodenum are recorded under isometric conditions.

This model enables evaluation of neurotropic (beta agonist) and musculotropic spasmolytic or spasmogenic effects

• Modulation of spontaneous contractions of the duodenum

Reference substances: Isoprenaline, Papaverine (in alphabetical order).

Ref ML3

Species : Guinea pig

Contractile activity of isolated rings of trachea is recorded under isometric conditions.

Isolated preparations of guinea pig trachea are used for evaluation of neurotropic (muscarinic, histaminic, beta₂-adrenergic) and musculotropic (calcium blockers, phosphodiesterase inhibitors) relaxant or contracting effects.

• Cholinergic, beta₂-adrenergic, histaminergic agonist or antagonist
• Musculotropic relaxant effect

Reference substances: Atropine, Carbachol, Histamine, Isoprenaline, Nicardipine, Papaverine, Pyrilamine, Salbutamol (in alphabetical order).

Ref ML4

Species : Rat

Contractile activity of isolated uterine horns is recorded under isometric conditions.

Isolated preparations of uterine horns are used for evaluation of neurotropic (oxytocin and beta₂-adrenergic mediated effects) and musculotropic relaxant or contracting effects.

• Effect on oxytocin mediated spontaneous uterine contractions or induced by exogenous oxytocin
• Musculotropic relaxant effect

Reference substances: Isoprenaline, Oxytocin, Papaverine (in alphabetical order).

Ref ML5

Species : Rat

Contractile activity of isolated vas deferens is recorded under isometric conditions.

Isolated preparations of vas deferens are used for evaluation of neurotropic (purinergic mediated effects) and musculotropic relaxant or contracting effects.

• alpha₁/alpha₂ -adrenergic agonist or antagonist effect

Reference substances: 2-chloro-adenosine, Theophylline (in alphabetical order).

Ref ML6

Species : Rabbit

Contractile activity of isolated urethra is recorded under isometric conditions.

Isolated preparations of urethra are used for evaluation of neurotropic (alpha₁-adrenergic mediated effects) and musculotropic relaxant or contracting effects.

• Alpha₁-adrenergic agonist or antagonist effect
• Musculotropic relaxant effect

Reference substances:Phenylephrine, Prazosin (in alphabetical order).

Ref ML7

Species : Rabbit

Contractile activity of isolated trigone is recorded under isometric conditions.

Isolated preparations of trigone are used for evaluation of neurotropic (alpha₁-adrenergic mediated effects) and musculotropic relaxant or contracting effects.

• Alpha₁-adrenergic agonist or antagonist effect
• Musculotropic relaxant effect

Reference substances: Phenylephrine, Prazosin (in alphabetical order).

Ref ML8

Species : Rabbit

Isolated preparations of corpus cavernosum are used for evaluation of smooth muscle relaxant effects of drugs that may be active in erectile dysfunction.

Relaxation of phenylephrine or KCl induced contractions

Reference substance:Sodium nitroprusside

Ref ML9

Species : Guinea pig

The model of isolated proximal colon of guinea pigs is a reliable model for investigating 5-HT4 receptor-mediated neurogenic excitatory responses in intestinal preparations.

• 5-HT4 agonist or antagonist effect

Reference substances: 5-methoxytryptamine.

Ref ML10

Species : Guinea pig

The model of isolated bladder of guinea pigs is used for evaluation of neurotropic (cholinergic mediated effects) and musculotropic relaxant or contracting effects.

• Cholinergic agonist or antagonist effect
• Musculotropic effect

Reference substances: Acetychloline, Atropine, Barium chloride, Papaverine (in alphabetical order).